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| Cata #: | Name of Product: | Price: |
| HRP-2813 | Recombinant Human UBE2B Protein | $300 |
| Product Name: | Recombinant Human UBE2B Protein |
| Catalog #: | HRP-2813 |
| Manufacture: | LD Biopharma, Inc. |
| Introduction: | Human Ubiquitin-Conjugating Enzyme E2 B (UBE2B) gene encodes a E2 enzyme which accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. It is also required for post-replication repair of UV-damaged DNA. UBE2B is associated with the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It may be involved in neurite outgrowth Full-length human UBE2B cDNA (151aa) was constructed with codon optimization gene synthesis and expressed with a human N-terminalT7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as soluble one. The final product was purified using affinity column. |
| Gene Symbol: | UBE2B (RAD6B; UBCH1; E2-17kDa; HHR6B; UBC2) |
| Accession Number: | NP_003328 |
| Species: | Human |
| Package Size: | 50µg / Vial |
| Composition: | 1.0 mg/ml, sterile filtered, in 20% Glycerol / PBS buffer. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Barnsby-Greer,L., et al.,UBE2A and UBE2B are recruited by an atypical E3 ligase module inUBR4. Nat Struct Mol Biol 31 (2), 351-363 (2024) Zeng,X., et al.,UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization. Bioengineered 13 (4), 8000-8012 (2022)
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| Quality Control: | Purity: > 92 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS |