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| Cata #: | Name of Product: | Price: |
| HRP-2265 | Recombinant Human MAGEA3-11R Protein | $300 |
| Product Name: | Recombinant Human MAGEA3-11R Protein |
| Catalog #: | HRP-2265 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human melanoma-associated antigen 3 (MAGEA3) gene encodes a tactic specific protein which was proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It may enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. MAGEA3 may act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3: substrate complex. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Recently MAGEA3 was functionally characterized as having a pro-tumor role in experimental models of pancreatic cancer and HCC. MAGEA3 mediates increased proliferation and chemore-sistance against cytotoxic agents in pancreatic and various HCC cell lines. Targeting MAGEA3 was demonstrated as unique strategy for potential HCC therapy when MAGES3 function as a driver of liver cancer progression. Full-length human MAGEA3 cDNA (313aa) was constructed with codon optimization gene synthesis and expressed with a human N-terminalT7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | MAGEA3 ( MAGE3 ) |
| Accession Number: | NP_005353 |
| Species: | Human |
| Package Size: | 50µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and other. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Amanda J.Craig, et al., Transcriptiomic characterization of cancer-testis antigen identifies MAGEA3 as a driver of tumor progression in hepatocellular carcinoma. PLOS Genetics 17(6): e1009589 (2021). Khalvandi A, et al.,Nuclear overexpression levels of MAGE-A3 predict poor prognosis in patients with prostate cancer. APMIS 129 (6), 291-303 (2021) Chen A, et al.,MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. PLoS One 15 (11), e0241551 (2020) |
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| Quality Control: | Purity: > 93 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFELPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHMVKISGGPHISYPPLHEWVLREGEEESGGGGSPGRRRRRRRRRRR |