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| Cata #: | Name of Product: | Price: |
| HTF-3208 | Recombinant Human TFEB-11R Protein | $300 |
| Product Name: | Recombinant Human TFEB-11R Protein |
| Catalog #: | HTF-3208 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Recent studies suggest that a number of genes encoding lysosomal proteins exhibit coordinated transcriptional behavior and are regulated by the transcription factor EB (TFEB). As transcription factor, TFEB specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. TFEB specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. TFEB plays a role in the signal transduction processes required for normal vascularization of the placenta. Full-length human TFEB (475aa, Isoform-1. derived from BC032448) gene was constructed with codon optimized gene synthesis technology, and fusion with 28 aa N-terminal T7/His /TEV cleavage site Tag and 11 arginine (11R tag) at C-terminal. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | TFEB (ALPHATFEB; BHLHE35; TCFEB) |
| Accession Number: | NP_009093 |
| Species: | Human |
| Package Size: | 40 µg / Vial |
| Composition: | 0.4 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT & Others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Rushika M.Perera, et al., Transcriptional control of autophage-lysosome function drives pancreatic cancer metabolism. Nature. Vol 524, 361-365 (2015) Magini A, et al., TFEB activation promotes the recruitment of lysosomalglycohydrolases beta-hexosaminidase and beta-galactosidase to theplasma membrane. Biochem. Biophys. Res. Commun. 440 (2), 251-257 (2013) Song W, et al., TFEB regulates lysosomal proteostasis. Hum. Mol. Genet. 22 (10), 1994-2009 (2013) Pastore N, et al., Gene transfer of master autophagy regulator TFEB results inclearance of toxic protein and correction of hepatic disease inalpha-1-anti-trypsin deficiency. EMBO Mol Med 5 (3), 397-412 (2013)
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| Quality Control: | Purity: > 91 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGFASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVLGGRESGGGGSPGRRRRRRRRRRR |