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| Cata #: | Name of Product: | Price: |
| HRP-3156 | Recombinant YFP-Human Rab11b Fusion Protein | $300 |
| Product Name: | Recombinant YFP-Human Rab11b Fusion Protein |
| Catalog #: | HRP-3156 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The human Ras-related protein Rab-11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. It may also regulate constitutive and regulated secretion, like insulin granule exocytosis. It is required for melanosome transport and release from melanocytes. Rab11B also regulates V-ATPase intracellular transport in response to extracellular acidosis. Recent data indicated that Rab11B mediated integrin recycling which promote brain cancer cells metastatic adaptation and outgrowth. Full-length mature form of human Rab11b cDNA (2 - 215aa) was constructed with codon optimization gene synthesis and expressed with YFP Protein as N-terminal (YFP; 256aa) fusion protein in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | Rab11b ( YPT3 ) |
| Accession Number: | NP_004209 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Howe, E.N., et al. Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.Nature Communi. doi.org/ 10.1038 /s41467-020-16832-2. (2020) Zulkefli KL, et al., A role for Rab11 in the homeostasis of the endosome-lysosomalpathway. Exp. Cell Res. 380 (1), 55-68 (2019) |
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| Quality Control: | Purity: > 90 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNDLYKGSENLYFQGEFGSGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC |