Invention in the REGENERATIVE MEDICINE is what we do every day, benefits of patients is our final goal
| Cata #: | Name of Product: | Price: |
| HTF-2939 | Recombinant YFP-Human IRF8-11R Protein | $300 |
| Product Name: | Recombinant YFP-Human IRF8-11R Protein |
| Catalog #: | HTF-2939 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Interferon regulatory factor 8 (IRF8) gene encodes a protein, which plays a role as a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). IRF8 also plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Full-length human IRF8 cDNA (426aa, derived from BC126247) was constructed with codon optimization gene synthesis and expressed with YFP Protein as N-terminal (YFP; 256aa) and 11 arginine (11R tag) at C-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | IRF8 ( H-ICSBP; ICSBP1 ) |
| Accession Number: | NP_002154.1 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Parnell GP, et al., The latitude-dependent autoimmune disease risk genes ZMIZ1 and IRF8regulate mononuclear phagocytic cell differentiation in response tovitamin D. Hum. Mol. Genet. 28 (2), 269-278 (2019) Gaillard C, et al., Identification of IRF8 as a potent tumor suppressor in murine acutepromyelocytic leukemia. Blood Adv 2 (19), 2462-2466 (2018) Zhang Y, et al., Altered expression of transcription factors IRF4 and IRF8 inperipheral blood B cells is associated with clinical severity andcirculating plasma cells frequency in patients with myastheniagravis. Autoimmunity 51 (3), 126-134 (2018) Ye L, et al., Interferon Consensus Sequence-Binding Protein 8, a Tumor Suppressor, Suppresses Tumor Growth and Invasion of Non-Small Cell Lung Cancer by Interacting with the Wnt/beta-Catenin Pathway. Cell. Physiol. Biochem. 51 (2), 961-978 (2018) |
| Applications: |
|
| Quality Control: | Purity: > 90 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNDLYKGSENLYFQGEFCDRNGGRRLRQWLIEQIDSSMYPGLIWENEEKSMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITVESGGGGSPGRRRRRRRRRRR |