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VRP-3077 Recombinant sfGFP-SARS-CoV-2 ORF10 Fusion Protein $150
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Recombinant sfGFP-SARS-CoV-2 ORF10 Fusion Protein

Product Name: Recombinant sfGFP-SARS-CoV-2 ORF10 Fusion Protein
Catalog #:  VRP-3077
Manufacture:  LD Biopharma, Inc.
Intruduction:

Coronaviruses are a group of enveloped positive-stranded RNA viruses that consist of four structural proteins including spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. The Orf10 of SARS-CoV is 38aa protein which may hijack host cell ubiquitination pathways for replication and pathogenesis. The novel Orf10 of SARS-CoV-2 interacts with members of a Cullin 2 (CUL2) RING E3 ligase complex, specifically the CUL2ZYG11B complex.

Full-length SARS-coV2 viral ORF10 cDNA (37aa) was constructed with codon optimization gene synthesis and expressed with a SuperGFP Protein N-terminal (sfGFP; 257aa) fusion at target protein N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. 

Gene Symbol:  SARS-Cov2 ORF10 
Accession Number:  QHI42199.1
Species:  Coronovirus
Package Size:  30 µg / Vial   
Composition: 0.3 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference:

Davld E. Gordon.et al., A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature https://doi.org/10.1038/s41586-020-2286-9 (2020) 

Wu, F. et al., 2020: A new coronavirus associated with human respiratory disease in China. Nature 579(7798): 265-269.

Applications:
  1. May be used for SARS-CoV2 ORF10 protein-protein interaction assay.
  2. As native SARS-CoV2 ORF10 immunogen for its specific antibody production.
Quality Control:
  • Purity: > 93 % by SDS-PAGE.
  • SuperGFP protein: Ex λ = 485, and Em λ = 510.
Recombinant Protein Sequence:

MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSGLRSGGSGGGEGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT

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