Human transcription factor AP-1 (also named as c-Jun) gene encodes a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Recent data indicated that c-Jun overexpression in CAR-T cells induces exhaustion resistance.

Full-length human C-Jun cDNA (330aa, derived from BC068522) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R) tag at its C-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

C-Jun               ( AP1, c-Jun; p39 )

Rachel C Lynn, et al., C-jun overexpression in CAR-T cells induces exhaustion resistance. Nature. https://doi.org/10.1038/s41586-019-1805-z (2019)

Addison WN, et al., Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast

 Differentiation. J. Biol. Chem. 294 (20), 8184-8196 (2019)

 Yoon H, et al., p27 transcriptionally coregulates cJun to drive programs of tumor progression. Proc. Natl. Acad. Sci. U.S.A. 116 (14), 7005-7014 (2019)

1. May be used for in vitro c-Jun mediated gene transcription regulation study in B cell or neuronal cell’s differentiation by intracellular delivery of this cJun-11R protein directly in vitro cell culture.
2. May be used for mapping c-Jun protein-protein interaction.
3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
4. Potential biomarker protein / therapeutic target protein for modulating CAR-T cell activities, such as overcome CAR-T cell exhaustion.
5. As native human c-Jun immunogen for specific antibody production.

Download Datasheet