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| Cata #: | Name of Product: | Price: |
| HTF-0052 | Recombinant Human Nanog-11R Protein | $300 |
| Product Name: | Recombinant Human Nanog-11R Protein |
| Catalog #: | HTF-0052 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | NANOG is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. This human protein is encoded by the NANOG gene. It is a marker of undifferentiated human embryonic stem cells and has been used to enhance formation efficiency of induced pluripotent stem (iPS) cells from human fibroblasts. Recombinant human NANOG-11R protein was constructed with C-terminal tag of 11 arginine domain, which will efficiently deliver protein intracellularly. This protein was expressed in E.coli, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in various culture media at 2 – 8 µg/ ml may help to increase PiPS generation efficiency when combined with proteins Oct4-11R, Sox2-11R, Klf4-11R and cMyc-11R. |
| Gene Symbol: | NANOG |
| Accession Number: | NP_079141.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.50 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days |
| Key Reference: | Hongyan Zhou, et al. Generation of induced pluripotent stem cells using recombinant protein. Cell Stem Cell. Vol 4. Issue 5: 381-384 (2009) Vladimir Torchilin. Intracellular delivery of protein and peptide therapeutics. Drug Discovery Todays: Technologies. 01.002 (2009) Junying Yu et al. Induced Pluripotent Stem cell lines Derived from human somatic cells. Science. 10.1126 (2007) |
| Applications: | 1. Protein transduction for enhancing PiPS generation efficiency. 2. Active protein, may be used for ELISA based DNA/Protein binding assay. 3. As specific protein substrate for kinase assay. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. 2. DNA binding activity was demonstrated with ELISA using NANOG specific DNA binding oligo. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDVESGGGGSPGRRRRRRRRRRR Download Datasheet |