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| Cata #: | Name of Product: | Price: |
| HRP-1842 | Recombinant Human ALPP Protein | $300 |
| Product Name: | Recombinant Human ALPP Protein |
| Catalog #: | HRP-1842 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by human alkaline phosphatase, placental type preproprotein (ALPP) gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. Mature form of human ALPP cDNA (23-506aa, derived from BC009647) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | ALPP (ALP; PALP; PLAP-1) |
| Accession Number: | NP_001623.3 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Vatin M, et al., Polymorphisms of human placental alkaline phosphatase are associated with in vitro fertilization success and recurrent pregnancy loss. Am. J. Pathol. 184 (2), 362-368 (2014) Ravenni,N., et al., A human monoclonal antibody specific to placental alkaline phosphatase, a marker of ovarian cancer. MAbs 6 (1), 86-94 (2014) Solomon AL, et al., Placental alkaline phosphatase de-phosphorylates insulin-like growth factor(IGF) binding protein-1. Placenta 35 (7), 520-522 (2014) Bellazi L, et al., A sequence variation in the promoter of the placental alkaline phosphatase gene (ALPP) is associated with allele-specific expression in human term placenta. Placenta 31 (9), 764-769 (2010) Lowe ME. Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein. J. Cell Biol. 116 (3), 799-807 (1992) |
| Applications: | 1. May be used for in vitro ALPP mediated Insulin-like growth factor (IGF) pathway regulation study in stem cell proliferation by coating this protein as matrix protein or use it as soluble factor. 2. May be used for protein-protein interaction mapping. 3. As enzymatic substrate for various proteases. 4. Potential biomarker protein for tumor diagnosis, such as ovarian cancer, et al. 5. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFIIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD Download Datasheet |