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| Cata #: | Name of Product: | Price: |
| hRP-1787 | Recombinant Human ATIA Protein | $300 |
| Product Name: | Recombinant Human ATIA Protein |
| Catalog #: | hRP-1787 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The regulation of apoptosis is critical for controlling tissue homeostasis and preventing tumor formation and growth. Reactive Oxygen Species (ROS) generation plays a key role in such regulation. Human ATIA (anti-TNFa-induced apoptosis) protects cells again TNFa and Hypoxia-induced apoptosis. ATIA gene is highly expressed in some tissues including liver, lung, heart, and testis. The human ortholog of ATIA, vasorin, was reported to be a potential inhibitor of TGFβ signaling with a possible role in angiogenesis. Recent data indicated that ATIA is highly expressed in human glioblastoma, and ATIA knockdown in glioblastoma cells renders them sensitive to hypoxia-induced apoptosis. Full-length extracellular domain of human ATIA cDNA (24 – 575aa, derived from BC068575) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | ATIA (VASN, vasorin) |
| Accession Number: | NP_612449.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Anning Lin, ATIA: A link between inflammation and hypoxia. Mol Cell. 42(5): 557-558 (2011). Swati Choksi, et al., A HIF-1 target, Protects cells from Apoptosis by Modulating the Mitochondrial Thioredoxin, TRX2. Mol Cell.42(5): 597-609 (2011). Ikeda Y, et al., Vasorin, a transforming growth factor beta-binding protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo. Proc. Natl. Acad. Sci. U.S.A. 101 (29), 10732-10737 (2004) |
| Applications: | 1. May be used for in vitro ATIA mediated TGFβ pathway regulation study for normal or cancer cells with recombinant ATIA protein either as soluble factor or as coating matrix protein. 2. May be used for protein-protein interaction mapping. 3. Potential diagnostic/therapeutic target for glioblastoma and other tumors. 4. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFCPSGCQCSQPQTVFCTARQGTTVPRDVPPDTVGLYVFENGITMLDAGSFAGLPGLQLLDLSQNQIASLPSGVFQPLANLSNLDLTANRLHEITNETFRGLRRLERLYLGKNRIRHIQPGAFDTLDRLLELKLQDNELRALPPLRLPRLLLLDLSHNSLLALEPGILDTANVEALRLAGLGLQQLDEGLFSRLRNLHDLDVSDNQLERVPPVIRGLRGLTRLRLAGNTRIAQLRPEDLAGLAALQELDVSNLSLQALPGDLSGLFPRLRLLAAARNPFNCVCPLSWFGPWVRESHVTLASPEETRCHFPPKNAGRLLLELDYADFGCPATTTTATVPTTRPVVREPTALSSSLAPTWLSPTEPATEAPSPPSTAPPTVGPVPQPQDCPPSTCLNGGTCHLGTRHHLACLCPEGFTGLYCESQMGQGTRPSPTPVTPRPPRSLTLGIEPVSPTSLRVGLQRYLQGSSVQLRSLRLTYRNLSGPDKRLVTLRLPASLAEYTVTQLRPNATYSVCVMPLGPGRVPEGEEACGEAHTPPAVHSNHAPVTQAREGNLP Download Datasheet |