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| Cata #: | Name of Product: | Price: |
| hRP-0913 | Recombinant Human ARFIP2 Protein | $300 |
| Product Name: | Recombinant Human ARFIP2 Protein |
| Catalog #: | hRP-0913 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | BAR(Bin/Amphiphysin/Rvs) domains form a curved helical homodimer that can associate with membranes to sense or induce curvature. Unlike other membrane-binding domains that recognize the head groups of specific phospholipids, BAR domains bind nonspecifically to acidic membrane surfaces via basic residues on their concave face. Arfaptins (Arfaptin-1 and Arfaptin-2) are canonical BAR domain-containing proteins with no additional lipid-binding modules. Arfaptin-2 (ARFIP2) alone can deform liposomes into tubules in vitro and can bind to the Arf and Rac GTPases, suggesting that membrane remodeling induced by Arfaptin-2 can be modulated by these GTPases. Recent data also indicated that ARFIP2 protein might plays a role in regulating huntingtin protein aggregation. Full-length human Arfaptin-2 (341aa, Isoform_II) gene was constructed with 15 aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | ARFIP2 (POR1) |
| Accession Number: | NP_036534 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Nakamura,K., et al., Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase. J. Biol. Chem. 287 (30), 25478-25489 (2012) Rangone,H., et al., Phosphorylation of arfaptin 2 at Ser260 by Akt Inhibits PolyQ-huntingtin-induced toxicity by rescuing proteasome impairment. J. Biol. Chem. 280 (23), 22021-22028 (2005) Peters,P.J., et al., Arfaptin 2 regulates the aggregation of mutant huntingtin protein. Nat. Cell Biol. 4 (3), 240-245 (2002) |
| Applications: | 1. May be used for in vitro ARFIP2 mediated membrane sense pathway regulation study by intracellular delivery of this protein with “ProFectin” reagent. 2. May be used for mapping ARFIP2 protein – protein interaction assay. 3. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays. 4. May be used for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGEFMTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSTTPSGPGDEVARGIAGEKFDIVKKWGINTYKCTKQLLSERFGRGSRTVDLELELQIELLRETKRKYESVLQLGRALTAHLYSLLQTQHALGDAFADLSQKSPELQEEFGYNAETQKLLCKNGETLLGAVNFFVSSINTLVTKTMEDTLMTVKQYEAARLEYDAYRTDLEELSLGPRDAGTRGRLESAQATFQAHRDKYEKLRGDVAIKLKFLEENKIKVMHKQLLLFHNAVSAYFAGNQKQLEQTLQQFNIKLRPPGAEKPSWLEEQ Download Datasheet |