Invention in the REGENERATIVE MEDICINE is what we do every day, benefits of patients is our final goal
| Cata #: | Name of Product: | Price: |
| HTF-1767 | Recombinant Human Sox5-11R Protein | $300 |
| Product Name: | Recombinant Human Sox5-11R Protein |
| Catalog #: | HTF-1767 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Sox5 gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The Sox5 may act as a transcriptional regulator after forming a protein complex with other proteins. It may play a role in chondrogenesis. Recent data indicated that SOX5 can activate the HMGB2 enhancer for subsequent gero-protective effects such as rejuvenation of aged knee joints through epigenetic remodeling (over-expression of human Sox5 gene) . Full-length human Sox5 cDNA (762aa, Isoform-I, derived from BC060773) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R) tag at its C-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | SOX5 ( L-Sox5; L-Sox5B; L-Sox5F ) |
| Accession Number: | NP_008871 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose ,DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Wang D, et al., SOX5 promotes epithelial-mesenchymal transition and cell invasionvia regulation of Twist1 in hepatocellular carcinoma. Med. Oncol. 32 (2), 461 (2015) Renjie W et al., MiR-132, miR-15a and miR-16 synergistically inhibit pituitary tumorcell proliferation, invasion and migration by targeting Sox5. Cancer Lett. 356 (2 PT B), 568-578 (2015) Mata-Rocha M, et al., The transcription factors Sox5 and Sox9 regulate Catsper1 geneexpression. FEBS Lett. 588 (18), 3352-3360 (2014) Yaobin Jing, et al., Genome-wide CRISPR activation screening in senescent cells reveals SOX5 as a driver and therapeutic target of rejuvenation. Cell Stem Cell. https://doi.org/10.1016/j.stem.2023.09.007 |
| Applications: |
|
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFSSKRPASPYGEADGEVAMVTSRQKVEEEESDGLPAFHLPLHVSFPNKPHSEEFQPVSLLTQETCGHRTPTSQHNTMEVDGNKVMSSFAPHNSSTSPQKAEEGGRQSGESLSSTALGTPERRKGSLADVVDTLKQRKMEELIKNEPEETPSIEKLLSKDWKDKLLAMGSGNFGEIKGTPESLAEKERQLMGMINQLTSLREQLLAAHDEQKKLAASQIEKQRQQMELAKQQQEQIARQQQQLLQQQHKINLLQQQIQVQGQLPPLMIPVFPPDQRTLAAAAQQGFLLPPGFSYKAGCSDPYPVQLIPTTMAAAAAATPGLGPLQLQQLYAAQLAAMQVSPGGKLPGIPQGNLGAAVSPTSIHTDKSTNSPPPKSKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQANESGGGGSPGRRRRRRRRRRR |