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hTF-0301 Recombinant Human NeuroD1-11R Protein $300
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Recombinant Human NeuroD1-11R Protein

Product Name: Recombinant Human NeuroD1-11R Protein
Catalog #:  hTF-0301
Manufacture:  LD Biopharma, Inc.
Intruduction:

Human NeuroD1 (Neurogenic differentiation factor 1) encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. Recent data also demonstrated that NeuroD1 play a important role for Trans-differentiation of human fibroblast cell to neuronal cells in vitro. Using mouse in vivo model, over-expression of NeuroD1 gene can convert reactive glial cell into functional neuronal cell for repairing brain injury.

Full-length human NeuroD1 cDNA (355 aa) was constructed with codon optimization gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal & 11 arginine Tag (11R Tag). This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  NeuroD1 (Beta2, BHF-1, bHFHa3, MODY6) 
Accession Number:  NP_002491
Species:  Human
Package Size:  40 µg / Vial   
Composition: 0.4 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Ziyuan Guo.,et al. In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer’s disease model. Cell Stem Cell. February 6; 14(2): 188-202. (2014)
Shimoda,M.,et al. Neurogenic differentiation 1 directs differentiation of cytokeratin 19-positive human pancreatic nonendocrine cells into insulin-producing cells Transplant. Proc. 42 (6), 2071-2074 (2010)
Zhiping P.Pang, et al. Induction of human neuronal cells by defined transcription factors. Nature May 26. doi: 10.1038/nature10202 (2011)
Hongyan Zhou, et al. Generation of induced pluripotent stem cells using recombinant protein. Cell Stem Cell. Vol 4. Issue 5: 381-384 (2009)
Applications:

1. May be used for in vitro NeuroD1 mediated gene transcription regulation study for neuronal cell differentiation by intracellular delivery of this protein

2. May be used for mapping NeuroD1 protein-protein interaction.

3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.

4. As Immunogen for specific antibody development.

Quality Control: Purity: > 90% by SDS-PAGE.
Recombinant Protein Sequence: MASMTGGQQMGRGHHHHHHGNLYFQGGEFTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEDDLEAMNAEEDSLRNGGEEEDEDEDLEEEEEEEEEDDDQKPKRRGPKKKKMTKARLERFKLRRMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSPDLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQNQDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFTMHYPAATLAGAQSHGSIFSGTAAPRCEIPIDNIMSFDSHSHHERVMSAQLNAIFHDLEESGGGGSPGRRRRRRRRRRR
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