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| Cata #: | Name of Product: | Price: |
| HRP-1127 | Recombinant Human IGFBP5 Protein | $150 |
| Product Name: | Recombinant Human IGFBP5 Protein |
| Catalog #: | HRP-1127 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human insulin-like growth factor-binding protein 5 (IGFBP5) gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. Recently, several studies have linked IGFBP5 to cancer metastasis, and have been associated with poor prognosis during metastasis. IGFBP5 has been demonstrated to interact directly with integrins a2b1, which plays a role in epithelial-mesenchymal transition. Full-length mature protein of human IGFBP5 cDNA (20-272aa, derived from BC011453) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | IGFBP5 (IBP5) |
| Accession Number: | NP_000590 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Lee,S.R., Influence of vascular endothelial growth factor on the expression of insulin-like growth factor-II, insulin-like growth factor binding protein-2 and 5 in human luteinized granulosa cells. Gynecol. Endocrinol. 28 (11), 917-920 (2012)
Sureshbabu,A., et al., IGFBP5 induces cell adhesion, increases cell survival and inhibits cell migration in MCF-7 human breast cancer cells. J. Cell. Sci. 125 (PT 7), 1693-1705 (2012) Peruzzi,B., et al., c-Src and IL-6 inhibit osteoblast differentiation and integrate IGFBP5 signalling. Nat Commun 3, 630 (2012) |
| Applications: | 1. May be used for in vitro IGFBP5 mediated IGF-independent activation of Akt activity regulation study through a2b1 integrin binding with this protein as either coating matrix protein or soluble factor. 2. May be used as IGFBP5 protein-protein interaction assay. 3. As enzymatic substrate for various proteases, such as , such as PAPP-A2, et al., 4. As antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE Dowaload Datasheet |