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| Cata #: | Name of Product: | Price: |
| HRP-1321 | Recombinant Human PD1 Protein | $150 |
| Product Name: | Recombinant Human PD1 Protein |
| Catalog #: | HRP-1321 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human programmed cell death protein 1 (PD-1, CD279) gene encodes a cell surface membrane protein of the immunoglobulin superfamily. CD279 protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for CD279 gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that CD279 gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. Full-length length human PD1 extracellular domain cDNA (21-170aa, derived from BC074740) was constructed with codon optimization and expressed with human Alpha-Fetal Protein (AFP) N-terminal domain Tag (1-198 aa) fusion at PD1 N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | PD1 (PDCD1; hPD-1; CD279; SLEB2) |
| Accession Number: | NP_005009.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Fourcade,J., et al., CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72 (4), 887-896 (2012)
Wiesmayr,S., et al., Decreased NKp46 and NKG2D and elevated PD-1 are associated with altered NK-cell function in pediatric transplant patients with PTLD. Eur. J. Immunol. 42 (2), 541-550 (2012) Li,M., et al., HBcAg induces PD-1 upregulation on CD4+T cells through activation of JNK, ERK and PI3K/AKT pathways in chronic hepatitis-B-infected patients. Lab. Invest. 92 (2), 295-304 (2012) |
| Applications: | 1. May be used for in vitro pro-B and T cells differentiation regulation study with this protein as either coating matrix protein or soluble factor. 2. May be used for protein-protein interaction assay. 3. As potential diagnostic biomarker protein for monitoring NK, B and T cell activities in vivo. ?4. As antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSNIEFPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLV Download Datasheet |