Human FoxD3 gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, which binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. It also acts as a transcriptional activator, promotes development of neural crest cells from neural tube progenitors. It restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. It is required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. Mutations in this gene cause autoimmune susceptibility 1.

Recombinant human FoxD3 protein was constructed with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly. This protein was expressed in E. coli, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in culture mediums at concentration of 2-8 ug/ml may be used for studying of human neuronal cell differentiation or maintaining ES cell self-renewal.

1. Protein transduction for human ES or neuronal cell differentiation.

2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay.

3. As specific protein substrate for kinase assay.

4. Immunogen for specific antibody production.

1. Purity: > 90% by SDS-PAGE.

2.DNA binding activity: to be tested.