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| Cata #: | Name of Product: | Price: |
| HTF-0120 | Recombinant Human MyoD1-11R Protein | $300 |
| Product Name: | Recombinant Human MyoD1-11R Protein |
| Catalog #: | HTF-0120 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human MyoD1 (myoblast determination protein 1) encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. Recombinant human MyoD1 protein was constructed with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly. This protein was expressed in E.coli, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in culture mediums at concentration of 2-8 ug/ml may be used for studying of human muscle cell differentiation. |
| Gene Symbol: | MyoD1 (bHLHc1; MYF3; MYOD; PUM) |
| Accession Number: | NP_002469 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days |
| Key Reference: | Voronova,A., et al. Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in viv0. Cell 66 (2), 305-315 (1991) Hongyan Zhou, et al. Generation of induced pluripotent stem cells using recombinant protein. Cell Stem Cell. Vol 4. Issue 5: 381-384 (2009) |
| Applications: | 1. Protein transduction for human myogenesis study. 2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay. 3. As specific protein substrate for kinase assay. 4. Immunogen for specific antibody production. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. 2.DNA binding activity: to be tested. |
| Recombinant Protein Sequence: | 29aa_Tag_ELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVLLEESGGGGSPGRRRRRRRRRRR Download Datasheet |